LOCUS       sequence-140638-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-BBa_B0012
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(134..161)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    198..217
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      220..263
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(267..285)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(513..532)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacatat aaacgcagaa aggcccaccc gaaggtgagc cagtgtgatc gatgagaccc
      181 atatgcgtct ctagattcta gaagcggccg cgaattccag aaatcatcct tagcgaaagc
      241 taaggatttt ttttatctga aattctgcct cgtgatacgc ctatttttat aggttaatgt
      301 catgataata atggtttctt agacgtcagg tggcactcga gttgatcggg cacgtaagag
      361 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
      421 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      481 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      541 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      601 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      661 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      721 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
      781 atttccggca gtttctacac atatattcgc a
//
