LOCUS       sequence-140639-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of mRIPK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  TITLE     Francis Chan lab plasmids
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-mRIPK3 WT-Flag/cHA-pTRIPZ vector
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(87..113)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(123..146)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(244..255)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cccttcgtct gacgtggcag cgctcgccgt gaggaggggg gcgcccgcgg gaggcgccaa
       61 aacccggcgc ggaggccacg cgtttaagcg taatctggaa catcgtatgg gtactcgagc
      121 tacttgtcat cgtcgtcctt gtagtccttg tggaagggct gccagcccct acccctgccg
      181 aactgtgctt ggtcatactt ggccgaactt gaggcagtag ttcttggtgg tgctaccaag
      241 gagttgtagt tcccaatctg cacttcagaa cagttgttga agacgagagc cggtggccct
      301 gtcattggat tcggtggggt ccagggatac caaggagtgc cgtgtcttcc atctccctga
      361 ttcctttggg ggtgaggacc gggagtctca gtaaagactg gcccaggtgt tgtgcctctg
      421 aagggtaaag tatgtggaat ctgaggagtg ccagccacgg ggtcagaaga tgtccttgct
      481 ggtgtggcag gcccaactga tgtgtcctgt gcttgcctct caggacattt tccaggaact
      541 ggtccggagg gttcctccaa atgcaggcgg tccagcattt tagaaaccat ggtttccctc
      601 gggcaatcca tttctgtgcc tctttggctt ggctctctgg cagacaagtt tctgccactg
      661 cttctgtgct gagacagata atgctttacc tcggagacag cagcatctac cttgtccttt
      721 accagattgt aa
//
