LOCUS       sequence-140640-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-BBa_B0015
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(134..161)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(177..248)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    286..305
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      308..351
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(355..373)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(601..620)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacatat aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga
      181 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
      241 tttatttgat gcctggtcga tgagacccat atgcgtctct agattctaga agcggccgcg
      301 aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg
      361 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      421 gcactcgagt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca
      481 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
      541 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      601 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
      661 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      721 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
      781 gtgatatggg atagtgttca cccttgttac accgttttc
//
