LOCUS       sequence-140643-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human GEC1/GABARAPL1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pGex-4T-2_GEC1
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(394..411)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(438..460)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgcgcgaggc agatcgtcag tcagtcacga tgcggccgct tatttcccat agacactctc
       61 atcactgtag gccacataca gaaaatagtc ttcctcatga ttgtcctcat acagttggcc
      121 catggtagca ctggtgggag ggatggtgtt gttgacaaag aagaataagg cgtcctcagg
      181 tctcaggtgg attctcttcc ggattaagaa gtagaactgg ccaacagtaa ggtcagaggg
      241 cactaggtac ttcctcttgt ccagatcagg caccctggct tttggagcct tctctacaat
      301 cacggggacc ctgtccggat atttcttccg gatcttttct ccttcctttt tccgatactc
      361 aaagggatgg tcctccttgt actggaactt catggatcca cgcggaacca gatccgattt
      421 tggaggatgg tcgccaccac caaacgtggc ttgccagccc tgcaaaggcc atgctatata
      481 cttgctggat ttcaagtact tatcaatttg tgggatagct tcaatacgtt ttttaaaaca
      541 aactaatttt gggaacgcat ccaggcacat tgggtccatg tataaaacaa catcaagagc
      601 gtcatacaac atgaagtcag gatgggttac atgatcacca tttaaatatg ttttatgaca
      661 taaacgatct tcgaacattt tcagcatttc aggtagcttg ctaagaaaat caactttgag
      721 agtttcaaag tctttactat atgcaattct cgaaacaccg tatctaatat ccaaaaccgc
      781 tccttcaagc attgaaatct ctgcacgctc ttt
//
