LOCUS       sequence-140645-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human LC3B/MAP1LC3B in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pET-15b_LC3B
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..410)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     CDS             complement(411..428)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(438..455)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             474..496
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    511..535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(535..554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(536..554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(638..657)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatcatc ctcgagcgcc gcttacactg acaatttcat cccgaacgtc
       61 tcctgggagg catagaccat gtacaggaat ccatcttcat ctttctcact ctcatacacc
      121 tctgagattg gtgtggagac gctgaccatg ctgtgtccgt tcaccaacag gaagaaggcc
      181 tgattagcat tgagctgtaa gcgccttcta attatcttga tgagctcact catgttgaca
      241 tggtcaggta caaggaactt tgttttatcc agaacaggaa gctgcttctc acccttgtat
      301 cgttctatta tcaccgggat tttggttgga tgctgctctc gaataagtcg gacatcttct
      361 actctttgtt cgaaggtgcg gcgctgcttg aaggtcttct ccgacggcat ggatccgcgc
      421 ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt atatctcctt
      481 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
      541 agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg
      601 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      661 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
      721 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
      781 gcggcggtgc tcaacggcct caacctacta ctg
//
