LOCUS       sequence-140647-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This binary vector, also named PTF101, is used as a standard plant 
            transformation vector. For more information, please see 
            http://plantsci.missouri.edu/muptcf/pzy101.html.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Zeng P, Vadnais DA, Zhang Z, Polacco JC
  TITLE     Refined glufosinate selection in Agrobacterium-mediated 
            transformation of soybean [Glycine max (L.) Merrill].
  JOURNAL   Plant Cell Rep. 2004 Feb;22(7):478-82. Epub 2003 Sep 30.
  PUBMED    15034747
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2004-02"; volume: "22(7)"; pages: "478-82. Epub 2003 
            Sep 30"
COMMENT     Sequence Label: pZY101
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    306..362
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     736..756
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
      361 ttgcatgccg gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga
      421 tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
      481 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
      541 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
      601 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
      661 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
      721 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
      781 tttggagag
//
