LOCUS       sequence-140648-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was derived from pZY101 by inserting a GUS-intron 
            reporter gene cassette into multiple cloning sites. This plasmid is 
            recommended for use as empty control for service event request..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Zeng P, Vadnais DA, Zhang Z, Polacco JC
  TITLE     Refined glufosinate selection in Agrobacterium-mediated 
            transformation of soybean [Glycine max (L.) Merrill].
  JOURNAL   Plant Cell Rep. 2004 Feb;22(7):478-82. Epub 2003 Sep 30.
  PUBMED    15034747
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2004-02"; volume: "22(7)"; pages: "478-82. Epub 2003 
            Sep 30"
COMMENT     Sequence Label: pZY102
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    179..200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(215..220,221..238,239..245)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    253..269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     258..280
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     277..293
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     277..293
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    302..358
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        448..792
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     736..756
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
       61 tgatccaagc tcaagctcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      121 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      181 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      241 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
      301 cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgc
      361 atgcctgcag gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
      421 tacagtctca gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa
      481 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
      541 agatggcttc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
      601 taccgacagt ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga
      661 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
      721 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
      781 tttggagagg acctcgactc tagaggatcc
//
