LOCUS       sequence-140657-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline inducible 3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Guan B, Wang TL, Shih IeM
  TITLE     ARID1A, a factor that promotes formation of SWI/SNF-mediated 
            chromatin remodeling, is a tumor suppressor in gynecologic cancers.
  JOURNAL   Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.
  PUBMED    21900401
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2011-11-1"; volume: "71(21)"; pages: "6718-27. Epub 
            2011 Sep 7"
COMMENT     Sequence Label: pLenti-puro
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             128..169
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             179..196
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(219..236)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(248..268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        266..563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        364..574
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      433..568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgactag tggatccagt
       61 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg
      121 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
      181 tcatcaccat caccattgag tttaaacctg ctgatcagcc tcgactgtgc cttctagtcg
      241 accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      301 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      361 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      421 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      481 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
      541 aagtagtgag gaggcttttt tggaggccta ggcttttgc
//
