LOCUS       sequence-140658-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of several 
            non-standard amino acids, into proteins in E coli in response to the
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAzFRS.2.t1
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttatcgca actctctact gtttctccat acccgttttt ttgggctaac aggaggaatt
       61 agatctatgg acgaatttga aatgataaag agaaacacat ctgaaattat cagcgaggaa
      121 gagttaagag aggttttaaa aaaagatgaa aaatctgctc tgataggttt tgaaccaagt
      181 ggtaaaatac atttagggca ttatctccaa ataaaaaaga tgattgattt acaaaatgct
      241 ggatttgata taattatatt gttggctgat ttacacgcct atttaaacca gaaaggagag
      301 ttggatgaga ttagaaaaat aggagattat aacaaaaaag tttttgaagc aatggggtta
      361 aaggcaaaat atgtttatgg aagtacttat atgcttgata aggattatac actgaatgtc
      421 tatagattgg ctttaaaaac taccttaaaa agagcaagaa ggagtatgga acttatagca
      481 agagaggatg aaaatccaaa ggttgctgaa gttatctatc caataatgca ggttaatggt
      541 tgtcattata ggggcgttga tgttgctgtt ggagggatgg agcagagaaa aatacacatg
      601 ttagcaaggg agcttttacc aaaaaaggtt gtttgtattc acaaccctgt cttaacgggt
      661 ttggatggag aaggaaagat gagttcttca aaagggaatt ttatagctgt tgatgactct
      721 ccagaagaga ttagggctaa gataaagaaa gcatactgcc cagctggagt tgttgaagga
      781 aatccaataa tggagatagc taaatacttc cttgaatatc ctttaaccat aaaagggcca
      841 gaaaaatttg gtggagattt gacagttaat agctatgagg agttagagag tttatttaaa
      901 aataaggaat tgc
//
