LOCUS       sequence-140667-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSIREN-RetroQ vector containing shRNA sequence to BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pSIREN-RetroQ-BPTF-sh3
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..138
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        267..766
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(302..325)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     699..718
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
       61 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      121 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      181 gaaaggacga ggatccgcga cgatgactcc gattatttca agagaataat cggagtcatc
      241 gtcgtttttt acgcgtgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
      301 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
      361 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
      421 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
      481 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
      541 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
      601 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
      661 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
      721 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gca
//
