LOCUS       sequence-140673-       12077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monitor post-translational degradation of Aiolos (Lentiviral, 
            SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12077)
  TITLE     Artichoke
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Aiolos in Artichoke
FEATURES             Location/Qualifiers
     source          1..12077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..33
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    150..171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(186..191,192..209,210..216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    224..240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..251
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     248..264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     248..264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     283..303
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        285..303
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        331..557
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             558..738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    785..910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1403..1636
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1821..1865
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2067..2473
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2511..2520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4095..4097,4098..4811)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4095..4097,4098..4808)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4137..4158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4398..4417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4745..4766
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4835..5409,5410..5412,5413..5421)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5002..5019)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5229..5248
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5431..5440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5437..6147
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5582..5600)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5850..5869
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6203..6320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6454..7632
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6685..7623
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7580..7600
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7645..8244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7645..7664)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8141..8161
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8260..8848
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8313..8333)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8731..8742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8920..9153
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9231..9352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9268..9287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9322..9341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9392..9527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9547..9566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9548..9566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9576..9593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9576..9592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9585..9607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9734..10189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9821..9840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10031..10052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10215..10319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10320..10388,10389..11180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10538..10557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11351..11939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11840..11859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
       61 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      121 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      181 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      241 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
      301 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
      361 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      421 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      481 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      541 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      721 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      781 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      841 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      901 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      961 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1021 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1081 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1141 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1201 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     1261 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1321 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1381 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1441 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1501 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1561 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1621 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1681 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1741 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1801 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1861 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1921 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1981 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct
     2041 attccagcac atatgagcta gctgcagtaa cgccattttg caaggcatgg aaaaatacca
     2101 aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc
     2161 aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca
     2221 ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc
     2281 tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc
     2341 ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc
     2401 ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgacaga
     2461 ctgagtcggc cggtcgaatc aagcttatcg ataccgtcga ctccggaata gccaccatgg
     2521 aagatataca aacaaatgcg gaactgaaaa gcactcagga gcagtctgtg cccgcagaaa
     2581 gtgcagcggt tttgaatgac tacagtttaa ccaaatctca tgaaatggaa aatgtggaca
     2641 gtggagaagg cccagccaat gaagatgaag acataggaga tgattcaatg aaagtgaaag
     2701 atgaatacag tgaaagagat gagaatgttt taaagtcaga acccatggga aatgcagaag
     2761 agcctgaaat cccttacagc tattcaagag aatataatga atatgaaaac attaagttgg
     2821 agagacatgt tgtctcattc gatagtagca ggccaaccag tggaaagatg aactgcgatg
     2881 tgtgtggatt atcctgcatc agcttcaatg tcttaatggt tcataagcga agccatactg
     2941 gtgaacgccc attccagtgt aatcagtgtg gggcatcttt tactcagaaa ggtaacctcc
     3001 tccgccacat taaactgcac acaggggaaa aaccttttaa gtgtcacctc tgcaactatg
     3061 catgccaaag aagagatgcg ctcacggggc atcttaggac acattctgtg gagaaaccct
     3121 acaaatgtga gttttgtgga aggagttaca agcagagaag ttcccttgag gagcacaagg
     3181 agcgctgccg tacatttctt cagagcactg acccagggga cactgcaagt gcggaggcaa
     3241 gacacatcaa agcagagatg ggaagtgaaa gagctctcgt actggacaga ttagcaagca
     3301 atgtggcaaa acgaaaaagc tcaatgcctc agaaattcat tggtgagaag cgccactgct
     3361 ttgatgtcaa ctataattca agttacatgt atgagaaaga gagtgagctc atacagaccc
     3421 gcatgatgga ccaagccatc aataacgcca tcagctatct tggcgccgaa gccctgcgcc
     3481 ccttggtcca gacaccgcct gctcccacct cggagatggt tccagttatc agcagcatgt
     3541 atcccatagc cctcacccgg gctgagatgt caaacggtgc ccctcaagag ctggaaaaga
     3601 aaagcatcca ccttccagag aagagcgtgc cttctgagag aggcctctct cccaacaata
     3661 gtggccacga ctccacggac actgacagca accatgaaga acgccagaat cacatctatc
     3721 agcaaaatca catggtcctg tctcgggccc gcaatgggat gccacttctg aaggaggttc
     3781 cccgctctta cgaactcctc aagcccccgc ccatctgccc aagagactcc gtcaaagtga
     3841 tcaacaagga aggggaggtg atggatgtgt atcggtgtga ccactgccgc gtcctcttcc
     3901 tggactatgt gatgttcacg attcacatgg gctgccacgg cttccgtgac cctttcgagt
     3961 gtaacatgtg tggatatcga agccatgatc ggtatgagtt ctcgtcgcac atagccagag
     4021 gagaacacag agccctgctg aaggctgaag ctgctgcaaa ggaagctgca gctaaggagg
     4081 ctgcagctaa ggctgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4261 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4321 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4501 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4561 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4681 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4801 tgtacaagta aatgcatgag taactgagga tccgcccctc tccctccccc ccccctaacg
     4861 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4921 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4981 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     5041 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     5101 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     5161 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     5221 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     5281 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
     5341 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5401 gatgataata tggccacaac cctggaattc gccaccatgg tgagcaaggg cgaggaggat
     5461 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     5521 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     5581 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     5641 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     5701 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     5761 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     5821 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     5881 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     5941 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     6001 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     6061 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     6121 ggcggcatgg acgagctgta caagtaaact agtaagcttg gcgtaactag atcttgagac
     6181 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     6241 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     6301 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttgggctc
     6361 gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     6421 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     6481 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     6541 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     6601 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     6661 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     6721 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     6781 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     6841 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     6901 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     6961 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     7021 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     7081 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     7141 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     7201 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     7261 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     7321 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     7381 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     7441 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     7501 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     7561 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     7621 caggtgtcgt gacgtacggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     7681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
     7861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     8041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8221 atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct ggattacaaa
     8281 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8341 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8401 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8461 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8521 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8581 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8641 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8701 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8761 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8821 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     8881 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8941 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     9001 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9061 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9121 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     9181 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9361 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9421 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9481 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9541 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9661 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9721 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9781 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9841 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9901 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9961 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10021 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10081 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10141 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    10201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11461 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    11521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12061 ataccgctcg ccgcagc
//
