LOCUS       sequence-140679-         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pTARBAC2 with kanaR selection marker and 
            lens-specific transgenesis marker dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-TarHom-Tol2-kanaR-cryaa-dsRed
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(191..214)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(389..408)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     606..626
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaata tggtatggct gattatgatc tagagtcgcg
      121 gccggccgct acaggaacag gtggtggcgg ccctcggcgc gctcgtactg ctccacgatg
      181 gtgtagtcct cgttgtggga ggtgatgtcc agcttggagt ccacgtagta gtagccgggc
      241 agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta gtggccgccg
      301 tccttcagct tcagggcctt gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
      361 cgctcggtgg aggcctccca gcccatagtc ttcttctgca ttacggggcc gtcggagggg
      421 aagttcacgc cgatgaactt caccttgtag atgaaggagc cgtcctgcag ggaggagtcc
      481 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      541 tcggggaagg acagcttctt gtagtcgggg atttcggcgg ggtgcttcac gtacaccttg
      601 gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg gcc
//
