LOCUS       sequence-140681-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pTARBAC2 with kanaR selection marker and 
            lens-specific transgenesis marker sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-TarHom-Tol2-kanaR-cryaa-sfGFP
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(136..849,850..852,853..855))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(181..202)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     789..810
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      852..861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
       61 gggaggtttt ttaaagcaag taaaacctct acaaatatgg tatggctgat tatgatctag
      121 agtcgcggcc ggccgctact tgtacagctc gtccatgccg tgagtgatcc cggcggcggt
      181 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcacgga
      241 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      301 ctgctggtag tggtcggcga gctgcacgct gccgtcctcc acgttgtggc ggatcttgaa
      361 gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgttgaa
      421 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      481 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtaggt
      541 gccgtcgtcc ttgaagctga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
      601 gaagaagtcg tggcgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
      661 cagggtggtc acgagggtgg gccaaggcac gggcagcttg ccggtggtgc agatgaactt
      721 cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg ccgcgcacgc tgaacttgtg
      781 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
      841 gcccttgctc accatggtgg cgaccggtgg atccacagta caaagatccc caaatgatgt
      901 gtgccggcct tagtggaggc agacccctgg ttatatacct gtgctgcaga aagaggggct
      961 tacaaatgga tctctctgga atggcattat ctcatggtgg g
//
