LOCUS       sequence-140683-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pTARBAC2 with kanaR selection marker and 
            lens-specific transgenesis marker sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-TarHom-Tol2-kanaR-cryaa-sfGFP
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(461..472,473..660)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
       61 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
      121 tgacgggacg gcggctttgt tgaataaatc gaactcttgc tgagttgaag gatcggatca
      181 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
      241 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg
      301 gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcggc
      361 ggccggatcc gatatcgttt ggcgcgccaa actcgctacc ttaggaccgt tatattaaga
      421 tccgatctgc tgggcttgct gaaggtaggg ggtcaagaac cagaggtgta aagtacttga
      481 gtaattttac ttgattactg tacttaagta ttatttttgg ggatttttac tttacttgag
      541 tacaattaaa aatcaatact tttactttta cttaattaca tttttttaga aaaaaaagta
      601 ctttttactc cttacaattt tatttacagt caaaaagtac ttattttttg gagatcactt
      661 cattctattt tcccttgcta ttaccaaacc aattgaattg cgctgatgcc cagtttaatt
      721 taaatgttat ttattctgcc tatgaaaatc gttttcacat tatatgaaat tggtcagaca
      781 tgttcattgg tcctttggaa gtgacgtcat gtcacatcta ttaccacaat gcacagcacc
      841 ttgacctgga aattagggaa attatatcag tcaatcagtg gaagaaaatg gaggaagtat
      901 gtgattcatc agcagctgcg agcagcacag tccaaaatca gccacaggat caagagcacc
      961 cgtggccgta tcttc
//
