LOCUS       sequence-140702-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for
            DSB mediated EGFP reconstitution. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
  TITLE     Generation of mutant mice by pronuclear injection of circular 
            plasmid expressing Cas9 and single guided RNA.
  JOURNAL   Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
  PUBMED    24284873
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-27"; volume: "3:3355. doi"; pages: " 
            10.1038/srep03355"
COMMENT     Sequence Label: pCAG-EGxxFP-Cetn1
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(114..135)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     463..482
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgagccaggg cattggccac accagccacc accttctgat aggcagcctg cacctgagga
       61 gtctcgagtt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      121 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      181 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      241 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      301 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      361 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      421 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      481 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      541 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
      601 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      661 agcttgccga tatcgaattc tgcaggcgca taggggctgg cgcaggcgca ctagcagggc
      721 acctcacaca cgtgcgatgt cagggcaccg ttaagatccc aactgttgtg gaggatggcg
      781 tccaccttca ggaagtcaaa cgttgcctct accagctaca agagaaaggt gggtcctaag
      841 cctgaactca ccgaagatca aaagcaagaa gttcgggaag cctttgacct cttcgattct
      901 gatgggagcg ggaccatcga tgtgaaggag ctgaaggtgg ccatgagagc acttggcttt
      961 gaacccagga aggaagagat gaagaaaatg atttcagaag tagacaa
//
