LOCUS       sequence-140704-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for
            DSB mediated EGFP reconstitution. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
  TITLE     Generation of mutant mice by pronuclear injection of circular 
            plasmid expressing Cas9 and single guided RNA.
  JOURNAL   Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
  PUBMED    24284873
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-27"; volume: "3:3355. doi"; pages: " 
            10.1038/srep03355"
COMMENT     Sequence Label: pCAG-EGxxFP-Cetn1
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(62..64,65..67,68..580)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(107..128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(368..387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaat ctagagccgc
       61 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctgaggatcc agtcacttaa taaaggttgg tctttttcat gatcttaaga aactcttcct
      721 cgttcacttc tccgtcgcca tcacgatcag cttcatcaat catttcctgc agctcctcgt
      781 ctgtgaggct ttcccccagc tcattggcca cacgcttgag gtttttgaat gaaattttcc
      841 cagtttcgtc gtcatcaaac aacctgaaag ccttcaggat ttcctctttg gtatctttct
      901 cggccatctt ctgagtcatc acagccaaga agtcattgaa gctgatcttt cctgtggcct
      961 ctttgtctac ttctgaaatc attttcttca tctcttcctt cctgggttca aa
//
