LOCUS       sequence-140705-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pRKVSV FAK
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..97
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             98..130
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             143..169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 cacaggtgtc cactcccagg tccaactgca cctcggttct atcgattcag ccgccaccat
       61 gggatacacc gacatcgaga tgaaccggct gggcaagtac accgacatcg agatgaaccg
      121 gctgggcaag ggatccacca tgtacgacgt tcctgattac gctagcctcc cgagatccgg
      181 tatggcagct gcttatcttg acccaaactt gaatcacaca ccaagttcga gtactaagac
      241 tcacctgggt actggcatgg aacggtcccc tggtgcaatg gaacgagtat taaaggtctt
      301 tcatcatttt gaaagcagta gtgagccaac cacctgggcc agtattatca ggcatggaga
      361 tgctactgat gtcaggggca tcattcagaa gatagtggac agtcacaaag taaagcacgt
      421 ggcctgctat ggatttcgcc tcagtcacct gcgatcggag gaggtgcact ggctgcacgt
      481 ggacatgggt gtctccagtg tgagggagaa atatgaactc gcccacccac cagaggagtg
      541 gaaatatgag ttgagaattc gttacttgcc aaaaggattt ctaaaccagt ttactgaaga
      601 taagccaaca ttgaatttct tctatcaaca ggtgaagagt gactacatgc aagaaatagc
      661 tgatcaagta gaccaagaaa tagctttgaa gttgggttgt ttggaaatta ggcgatccta
      721 ttgggagatg aggggtaatg ccctagagaa gaagtccaac tatgaagtat tagaaaaaga
      781 tgttggttta aagcgatttt ttcctaagag tttactggat tctgtcaagg ccaaaacact
      841 aagaaagctg atccagcaaa cattcaggca gtttgccaac cttaatagag aagaaagcat
      901 cctgaaattc tttgagattc tttctcctgt gtacagattt gacaaagagt gcttcaagtg
      961 tgcccttggg tcaagttgga tcatttctgt ggaattggca atcggcccag aagaagggat
     1021 cagttacctg acagacaaag gctgcaatcc cacacaccta gcagacttta acc
//
