LOCUS       sequence-140707-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMA7CR_2.0 contains arabinose inducible lambda/RED beta-protein and 
            dam, and aTc inducible CRISPR/Cas9 and recX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Ronda C, Pedersen LE, Sommer MO, Nielsen AT
  TITLE     CRMAGE: CRISPR Optimized MAGE Recombineering.
  JOURNAL   Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452.
  PUBMED    26797514
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-01-22"; volume: "6:19452. doi"; pages: " 
            10.1038/srep19452"
COMMENT     Sequence Label: pMA7CR_2.0
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             681..692
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    732..750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(769..796)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcaataaaag agcccctata agattttttt tgatactgtg gcggtctgta tttcccagaa
       61 ccttgaactt tttagacgga accttatatt catcagtgat caccgcccat ccgacgctat
      121 ttgtgccgat atctaagcct attgagtatt tcttatccat gtatatctcc ttctcagtcg
      181 gcaaaatttc gccaaatctc ctggatatct tccatcagat agccacgata gagcagaaaa
      241 cgctggatct taactttttc tgaaaagaca gttggcaaag gttcgccata ttttcgcgtc
      301 gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc
      361 gcttcgcggg aaataccttt ctgattcagt tcctggcgaa tacgcgcagg tccataacct
      421 ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag atagccatgt
      481 tcatggcacc aggcaataac gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg
      541 ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg
      601 cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg gcgacgggat
      661 gttgattctg tcatggtacc tttctcctct ttaatgaatt cggtcagtgc gtcctgctga
      721 tgtgctcagt atctctatca ctgataggga atagggagta gaaacgcaaa aaggccatcc
      781 gtcaggatgg ccttctgctt aatttgatgc ctggcagttt
//
