LOCUS       sequence-140711-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..254
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6..26
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     177..196
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
       61 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      121 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      181 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      241 aaggacgaaa caccggagac ggttgtaaat gagcacacaa aatacacatg ctaaaatatt
      301 atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat
      361 aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa
      421 cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt
      481 gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact
      541 ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct
      601 tcaaatgttg cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct
      661 tcaacagcta tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata
      721 atacatttaa caacatgtga tccagctgct ttttt
//
