LOCUS       sequence-140712-         558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    285..402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(458..533)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
       61 gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
      121 tctgcccact gacgggcacc ggagccaatt cccactcctt tcaagaccta gaaggtccat
      181 tagctgcaaa gattcctctc tgtttaaaac tttatccatc tttgcacccg ggccccctcg
      241 agccggcgcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
      301 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
      361 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
      421 atttgtctca agatctagtt acgccaagct taaaaaagca ccgactcggt gccacttttt
      481 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aacagagacg
      541 tacaaaaaag agcaagaa
//
