LOCUS       sequence-140714-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Tag_linker
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      171..228
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(252..269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(503..522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc ataggtctca gtactttaac
      121 tttaagaagg agatatataa aagagaccca tgccgtctca ttagctgcag tccggcaaaa
      181 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
      241 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      421 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt ga
//
