LOCUS       sequence-140720-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Strep(II)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             117..140
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      177..234
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(258..275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(509..528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc ataggtctca taaatgtgga
      121 gccacccgca gttcgaaaaa ccgcataaga gacccatgcc gtctcattag ctgcagtccg
      181 gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
      241 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      421 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      841 tggtggccta actacggcta cactaga
//
