LOCUS       sequence-140735-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_M5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 105 to 139)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL7
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        105..139
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(224..252)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaac gtctcaatct ctatttgacg gctagctcag
      121 tcctaggtac agtgctagcg gtctcagtac agatctaata attttgttta actttagggg
      181 aatacatatg agaccgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      241 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      301 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      361 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      421 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      481 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      541 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      601 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      661 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      721 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      781 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaa
//
