LOCUS       sequence-140750-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multi-purpose rapid titer bacmam expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Morales-Perez CL, Noviello CM, Hibbs RE
  TITLE     Manipulation of Subunit Stoichiometry in Heteromeric Membrane 
            Proteins.
  JOURNAL   Structure. 2016 Mar 24. pii: S0969-2126(16)00082-4. doi: 
            10.1016/j.str.2016.03.004.
  PUBMED    27041595
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2016 Mar 24. pii: S0969-2126(16)00082-4. doi: 
            10.1016/j.str.2016.03.004."
COMMENT     Sequence Label: pEZT-BM
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(22..732,733..735))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     413..432
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(760..869)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 cggttccgca tgctatgcat ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
       61 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
      121 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      181 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      241 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
      301 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      361 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      421 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      481 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
      541 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      601 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      661 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
      721 ctcgcccttg ctcaccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt
      781 tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata
      841 atatattgtg ttgggttgaa ttaaaggtcc gtatactccg gaatattaat aggatcccgg
      901 tccga
//
