LOCUS       sequence-140753-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APEX2 fused to Venus for transsynaptic tracing and the EM 
            analysis of infected neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-APEX2-Venus
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(27..53)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(60..806)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(807..830)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      830..839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cgaccggaat tccgtccagg gtcaggcgct ccaggggagg cagctgcagg
       61 gcatcagcaa acccaagctc ggaaagcttt tggtgagcct cagcgtaatc agcaaagaag
      121 gcatcttcgt ccgctgcata tttgtcaacg agagggcgga atacagggtc agacaaaaga
      181 gccttgtcag aaggtagctg aaggagacct tccttctcac cactcaacaa ctccgtgaag
      241 tatgagttgt cgaaaataag aggattagag gtccagggac cctcaaatcc agaacgctcc
      301 ttgtgtgcag ctccaatagt gtgaccccca gatagagcaa cgatatcttg gtcagtaagc
      361 cccatagctt tgccaaacac atctctcaaa tggtcagaac ccttagtggg atcgggcaag
      421 cgaccctctg gtggtggctc aggcttgtcc tctcttccag ggtggaatgg aaccttaggt
      481 ccacccgtga cctcaacggc aacaacgcca gccaactggt agaaatcggc gtagctcaaa
      541 atagggaact ccgccttgag tggctccaaa agcctaacag cgatgtcaag accgttgtta
      601 gcgctgtgag ccagttcggc agggtgcttg atggttccga agggtccacc ggtcttcgtg
      661 cccttgtcaa aggttccagc agagtggaat gccaaacgga gcattagagg agcgcatctc
      721 ttctcagcga tgaagcctct gagcttcttc ttcgccttct caacggcgtc ctggtaatca
      781 gcactcacag ttgggtaaga ctttccctta tcgtcgtcat ccttgtagtc catggtggc
//
