LOCUS       sequence-140761-       15732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses channelrhodopsin fused to YFP for monosynaptic circuit 
            mapping and optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 15732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-hChR2-YFP
FEATURES             Location/Qualifiers
     source          1..15732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4251
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      4261..4270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4267..4269,4270..4272,4273..4986)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4312..4333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4573..4592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4920..4941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7161..7172
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8964..8975)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12172..12190)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12186..12233
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12261..12277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12261..12277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12274..12296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12285..12301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12309..12315,12316..12333,12334..12339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12354..12375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12492..12509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12663..13251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12743..12762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13422..14213,14214..14282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14045..14064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14283..14387)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14414..14869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14501..14520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14711..14732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14995..15017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15009..15026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15010..15026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15096..15399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15400..15603
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15554..15574
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15648..15667
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15648..15666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggac tatggcggcg ctttgtctgc cgtcggacgc
     3361 gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat
     3421 caatgttact gtgccggatg gattgaatct cgcggcacga acggcgctca gaccgcgtca
     3481 aatgtcctgc agtggcttgc agcaggattc agcattttgc tgctgatgtt ctatgcctac
     3541 caaacctgga aatctacatg cggctgggag gagatctatg tgtgcgccat tgaaatggtt
     3601 aaggtgattc tcgagttctt ttttgagttt aagaatccct ctatgctcta ccttgccaca
     3661 ggacaccggg tgcagtggct gcgctatgca gagtggctgc tcacttgtcc tgtcatcctt
     3721 atccgcctga gcaacctcac cggcctgagc aacgactaca gcaggagaac catgggactc
     3781 cttgtctcag acatcgggac tatcgtgtgg ggggctacca gcgccatggc aaccggctat
     3841 gttaaagtca tcttcttttg tcttggattg tgctatggcg cgaacacatt ttttcacgcc
     3901 gccaaagcat atatcgaggg ttatcatact gtgccaaagg gtcggtgccg ccaggtcgtg
     3961 accggcatgg catggctgtt tttcgtgagc tggggtatgt tcccaattct cttcattttg
     4021 gggcccgaag gttttggcgt cctgagcgtc tatggctcca ccgtaggtca cacgattatt
     4081 gatctgatga gtaaaaattg ttgggggttg ttgggacact acctgcgcgt cctgatccac
     4141 gagcacatat tgattcacgg agatatccgc aaaaccacca aactgaacat cggcggaacg
     4201 gagatcgagg tcgagactct cgtcgaagac gaagccgagg ccggagccgt gccagcggcc
     4261 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4321 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4381 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4441 cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     4501 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4561 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4621 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4681 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4741 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4801 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4861 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4921 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4981 aagtaagcta gcggcgccta gccggtcatc cttttgacga ttccagtccc gaggataacc
     5041 tcctctcggg attgggggga atctttggat ccagtagtcc tccttgaact ccgtccaaca
     5101 gggcagattc aagagtcata agactttcat taatcatttc agttgatcag acatggtcgt
     5161 gtagattctc ttaatacggg agatcttcta gcagtttcag tgaccaacgg tgctttcatt
     5221 ctccaggaac tgataccaaa ggttgtggac aggccaaggg gtacttcgga tgactctgtg
     5281 cttgggcaca gaaagaggtc gtagtgtgcc ccccgatagc ggactcgaca tgaatcaact
     5341 aagaaaggca atctgcctcc catgatggac ataagcaata gttcacaacc atcttgcatc
     5401 tcagtgaagt gtacataact attgagggct gggtcatcta agcatttcag tcgagaaaaa
     5461 aactgtagac caaaagaaca actagcaaca cttctcatcc agagacccat atcaagatgc
     5521 tagatccggg agaggtttat gatgacccta ttgatccaat tgagtcagag gctgaaccca
     5581 gaggaacccc cactgtcccc aacatcttga ggaactccga ctacaatctc aattctcctt
     5641 tgatagagga tcctgccaaa ctaatgttag aatggttgaa gacagggaac agaccttatc
     5701 ggatgacttt gacagacaat tgctccaggt cttacaaagt tttgaaagat tatttcaaga
     5761 aagtagattt gggttctctc aaagtgggcg gaactgctgc acagtcaatg gtttctctct
     5821 ggttgtgtgg tgcccactct gaatcaaaca ggagccggag atgtataacc gacttggccc
     5881 atttctattc caagtcatcc cccatagaga agctattgaa ttgtacgcta ggaaacagag
     5941 gcctgagaat cccaccagag ggggtgttaa attgcctcga gagggtcaat tatgacaagg
     6001 catttgggag gtatctggcc aacacgtatt cctcttactt gtttttccat gtaatcacct
     6061 tatacatgaa tgccttagac tgggaagagg aaaagaccat cctggcatta tggaaagata
     6121 taacctcagt ggataccgag aaggacttgg tcaaattcaa agatcaaata tggggactgt
     6181 tgattgtgac aaaggacttt gtttactctc agagttcaaa ctgtcttttt gacagaaact
     6241 acacactgat gctaaaggat cttttcttgt ctcgattcaa ctccttaatg attctgcttt
     6301 ctccccctga gccccgatac tcagatgact taatatctca gctgtgccag ctatacatcg
     6361 ctggggatca agtcttgtcc atgtgtggga actccggcta tgaagtcatc aaaatattgg
     6421 agccatatgt cgtgaacagt ttggtccaga gggcagagaa gtttaggcct ctcatccacc
     6481 ccttgggaga ctttcctatg ttcataaaag acaaggtgaa tcaacttgaa gggacttttg
     6541 gtcccagtgc aaagaggttt tttagggttt tagatcaatt cgacaacata catgacttag
     6601 tatttgtgta tggctgttac agacattggg ggcaccccta tatagattat cggaagggtc
     6661 tgtcgaaact atatgatcaa gttcacatta agaaagtaat agataagtcc taccaggagt
     6721 gtttagcaag tgacttggcc agaaggatcc tcagatgggg atttgacaag tactccaaat
     6781 ggtatctaga ttcgagattc cttgcccgag accacccctt gactccttat gtcaagaccc
     6841 aaacatggcc acccaaacat atagtagact tggtggggga cacatggcat aagctcccga
     6901 tcacgcagat ctttgaaatt cctgaatcaa tggacccgtc agagatactg gatgacaaat
     6961 cacattcttt caccagaaca aggttagctt cttggctgtc agagaaccga ggggggcctg
     7021 ttcctagcga gaaggtcatt atcacggccc tgtctaagcc acctgtcaat ccccgagagt
     7081 ttttgaaatc tatagacctc ggaggattgc cagatgagga tttgataatt ggcctcaaac
     7141 caaaggaacg ggagttgaag atcgagggcc gattctttgc tctaatgtca tggaatctaa
     7201 gattatattt tgtcatcacc gaaaagctct tggccaacta cattttgcca ctttttgacg
     7261 cactgactat gacagacaac ctgaacaagg tgttcaaaaa gttgatcgac agggtcaccg
     7321 ggcaagggct tttggactat tctagggtca catacgcatt tcacctggac tatgagaaat
     7381 ggaacaatca tcaaagattg gagtcaacag aggatgtatt ctctgtccta gatcaggtgt
     7441 ttggattgaa gagggtgttt tctagaacac acgagttttt tcagaagtcc tggatctatt
     7501 attcagacag atcagacctc attgggttat gggaggacca aatatattgc ttggatatgt
     7561 ctaacggccc aacctgctgg aatggccaag atggcgggct agagggcttg cggcagaagg
     7621 gctggagtct agtcagttta ttaatgatag atagagaatc tcaaaccagg aacacaagaa
     7681 ccaagatact agctcaagga gacaaccagg ttctgtgtcc tacatacatg ttgtcaccgg
     7741 gattgtctca agaggggctt ctctatgagt tagagagcat atcaaggaat gcactctcaa
     7801 tataccgagc tatcgaggaa ggagcatcta agctggggct gatcatcaag aaggaagaga
     7861 ccatgtgtag ttatgacttt ctcatatatg ggaagacccc cttatttcga ggcaacatat
     7921 tggtgcctga atccaaaaga tgggcccgag tctcttgcat ctctaacgac caaatagtca
     7981 acctcgccaa tataatgtcg acagtatcca ccaatgcgct gacagtggca caacactctc
     8041 aatctctgat caaacctatg agggattttc tgctcatgtc agtacaggca gttttccact
     8101 acctgttgtt tagcccaatc ctaaaaggca gagtttataa gattctgagt gctgaagggg
     8161 agagctttct cctagccatg tcgcggataa tctacctaga tccttctttg ggaggggtgt
     8221 ctggaatgtc tctcgggagg ttccatatac gtcagttctc agaccctgtc tctgaagggt
     8281 tgtcattctg gagagagatc tggttaggct ctcatgagtc ctggattcac gcgttgtgtc
     8341 aggaggccgg gaaccccgat cttggagaga gaacactcga gagcttcact cgccttttag
     8401 aagatcctac taccttaaat atcaaaggag gggccagccc taccattcta ctcaaggatg
     8461 ctatcagaaa ggctctgtac gacgaggtgg acaaggtgga aaattcagag tttcgagagg
     8521 caatcctgct gtccaagacc catagagata actttatact ctttttaaaa tctgttgagc
     8581 ctctgttccc tcgatttctc agtgagctct tcagttcgtc tttcttggga ataccggagt
     8641 caatcattgg actgatacaa aactcccgga caataagaag gcagtttaga aagagtctct
     8701 caagaacttt agaagagtcc ttctacaact cagagatcca cgggattaat cggataaccc
     8761 agacacctca aagggtcgga agggtgtggc cttgctcttc agagagggcg gatctactta
     8821 gggagatctc ttgggggagg aaagtggtag gcacgacagt tcctcaccct tccgagatgt
     8881 tggagttgtt tcccaaatcc tccatttcct gcacttgtgg agcaacaggg ggaggcaatc
     8941 ctagagtctc tgtatcagta ctcccgtcct tcgatcagtc atttttctca cggggccccc
     9001 taaagggata cttgggctcg tccacctcta tgtcaaccca gctattccat gcatgggaaa
     9061 aagtcactaa tgttcatgtg gtgaaaaggg ctctatcgtt aaaagaatct ataaactggt
     9121 tcatcaatag gaattccaat ttggctcaaa ctctaattgg aaacatcatg tctctgacag
     9181 gccctgactt ccctctagaa gaggcccctg ttttcaaacg gacagggtca gccttgcata
     9241 ggttcaagtc tgccagatac agcgaaggag ggtattcttc tgtttgccct aaccttctct
     9301 cccatatctc tgttagtaca gacactatgt ctgatttgac ccaaaacggg aagaactatg
     9361 atttcatgtt tcagccattg atgctttatg cgcaaacatg gacatcggaa ctggtacaga
     9421 gggatacaag acttagagac tccacgtttc actggcacct tcggtgcaac agatgtgtga
     9481 ggcccattga tgatataaca ctggaaactt ctcagatctt cgagttcccg gatgtgtcaa
     9541 aaaggatatc caggatggtt tctggagctg tccctcaatt tcagaagctt cctgatatcc
     9601 gtctaagacc aggtgatttt gaatccctaa gtggtagaga aaagtctcgc catatagggt
     9661 cagctcaggg gctcttatac tcaatcttag tagcaattca cgactcagga tacaatgatg
     9721 ggaccatctt ccctgtcaac atatacggca aagtttcccc cagagactat ttgagagggc
     9781 ttgcaagagg gatcttgata gggtcctcga tttgcttctt gacacgaatg acaaatatta
     9841 acattaacag acctcttgaa ttgatctcag gggtaatttc ctatattctc ctgaggctgg
     9901 ataatcatcc ctctctgtat ataatgctca gagaaccgtc tcttagagga gaaatattct
     9961 ctatccctca gaaaatcccc gccgcttacc caaccactat gagagaaggc aacagatcga
    10021 ttttgtgtta cctccaacac gtgctacgct atgagcgaga ggcaatcacg gcgtccccgg
    10081 agaatgactg gctgtggatc ttctcagact tcagaagtgt gaaaatgacg tacttgaccc
    10141 tcattactta ccagtctcac ctcctactcc agagggttga gagaaacttg tctaagagta
    10201 tgagagctac tctgcgacaa atgggttcct taatgaggca agtgctgggt gggcacggag
    10261 aagatacctt ggagtcagac gatgacattc aacgattact aaaagactct ttgcgaaaga
    10321 caaggtgggt ggatcaagag gtgcgccatg cagctagaac catgaatgga gattacagcc
    10381 ccgacaagaa agtatcccgt aaggtaggat gttcagaatg ggtctgctct gctcaacaga
    10441 ttgccgtctc cacctcagcc aacccggccc ctgtctcaga gcttgacatt agggccctct
    10501 ctaagcggtt tcaaaacccc ttgatctcgg gcctgagagt ggttcagtgg gcaaccggtg
    10561 cccattataa gcttaagcct attctagatg atctaaatgt tttcccatct ctctgtcttg
    10621 taattggaga cgggtcaggg ggaatatcaa gggcagttct caacatgttt ccagattcta
    10681 agcttgtgtt caacagccta ttggaggtga atgatctgat ggcttccgga acacatccac
    10741 tgcctccttc agcaatcatg agtggaggag atgacatcat ctccagagtg atagactttg
    10801 actcaatatg ggagaaaccg tccgacctga ggaactcggc cacctggaga tacttccagt
    10861 cggttcaaaa acaggtcaac atgtcgtatg acctcattat ttgtgatgca gaagttactg
    10921 atattgcatc tatcaaccgg ataactctgt tgatgtctga tttcgcattg tctatagatg
    10981 gaccacttta tctggtcttc aaaacttacg ggactatgct agtaaacccg gactataaag
    11041 ctattcaaca tctgtcaaga gcgttccctt cggtcacagg gtttgtaacc caagtaactt
    11101 catccttttc ttctgagcta tacctccggt tctctaaacg aggaaagttt ttcagggacg
    11161 ccgagtactt gacctcttcc acccttcgag agatgagcct tgtgttgttc aattgcagca
    11221 gccccaaaag tgagatgcag agagctcgtt ccttaaacta tcaagacctg gtaaggggat
    11281 ttcctgaaga gatcatatca aatccttaca acgaaatgat cataactctg attgacaatg
    11341 atgtagagtc cttcctagtc cacaagatgg tggatgatct tgagctacag aggggaactc
    11401 tgtctaaagt ggctatcatt atatccatca tgatcgtttt ttccaataga gtcttcaaca
    11461 tttccaaacc tttgactgac cccttgttct atccaccgtc tgatcctaaa atcctgaggc
    11521 acttcaacat atgttgcagt actatgatgt atctatctac cgctttaggc gacgtcccta
    11581 acttcgcaag acttcatgac ctgtataaca gacctataac ttgttacttc agaaaacaag
    11641 ttattcgagg gaatatttat ctatcttgga gttggtccga tgatacccca gtgttcaaga
    11701 gagtagcctg taattctagc ttgagtctgt catctcactg gatcaggttg atctacaaga
    11761 tagtgaagac taccagactc attggcagca taaaagacct atcaggagag gtagaacgac
    11821 atcttcatgg gtataacaga tggatcaccc tcgaggatat ccgatctaga tcatccctac
    11881 tagattacag ttgtttgtaa gccggatatt accgaaagcc tgtgcatgct aaaattcttg
    11941 tatgatgcat cttgaaaaaa acaagatctt gaatccggac ctctggttgt ttgattgttt
    12001 tttccatctt tattgttttt ttgttaagcg tgggtcggca tggcatctcc acctcctcgc
    12061 ggtccgacct gggcatccga aggaggacgc acgtccactc ggatggctaa gggaggggcg
    12121 gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
    12181 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
    12241 tcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    12301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    12361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    12421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    12481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    12541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    12601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    12661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    12721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    12781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    12841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    12901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    12961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    13021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    13081 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    13141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    13201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    13261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    13321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    13381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    13441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    13621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    14041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    14101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    14161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    14221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    14281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    14341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    14401 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    14461 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    14521 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    14581 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    14641 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    14701 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    14761 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    14821 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat
    14881 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    14941 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    15001 cacgacgttg taaaacgacg gccagtgagc gcgcctagtt attaatagta atcaattacg
    15061 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    15121 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    15181 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    15241 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
    15301 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
    15361 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
    15421 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
    15481 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
    15541 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
    15601 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
    15661 tatagggaga cccaagctgg ctagattaag cgtctgatga gtccgtgagg acgaaacccg
    15721 gcgtaccggg tc
//
