LOCUS       sequence-140770-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nabi2.226 is a FRET (Forster Resonance Energy Transfer)-based 
            protein voltage sensor. This probe contains Clover and mRuby2 
            inserted at different locations in the Ciona voltage sensitive 
            domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ
  TITLE     Developing Fast Fluorescent Protein Voltage Sensors by Optimizing 
            FRET Interactions.
  JOURNAL   PLoS One. 2015 Nov 20;10(11):e0141585. doi: 
            10.1371/journal.pone.0141585. eCollection 2015.
  PUBMED    26587834
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-20"; volume: "10(11):e0141585. doi"; pages: " 
            10.1371/journal.pone.0141585. eCollection 2015"
COMMENT     Sequence Label: Nabi2.226
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    115..339
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     328..345
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
       61 agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca gaagccatag
      121 agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct
      181 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
      241 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
      301 aggggcaaac aacagatggc tggcaactag aaggcacagt cgagggcgcg cctattactt
      361 gtacagctcg tccatcccac caccaagccc ggcgaacttg gcaactgcgt gttcgcgttg
      421 tactacgaac atttcattgt cactttcctc taacctttcc aggcggtgat caacggcatg
      481 gataccgggc atcttgatgt tcccgacggt cttttttgac ctgtaagttg ttacgaaaga
      541 gcaagacaga tggccaccac catcaacttt cagtgccata tgagtgtatc ccctcagacc
      601 accatctgct ggatacatca tctctgtatt aggctcccaa cccttggtct tcttctgcat
      661 cacgggacca ttggagggaa agtttacccc tctgacttgg acgtggtaaa cgagacagcc
      721 atcctcaagg ctggtgtcct gcatgacggt gacgactcca ccatcttcgt atctcgtaac
      781 tctttcccaa gtaaaaccct caggaaagga ctgtttaaag aaatcaggaa tgcctttcgg
      841 gtacttgata aaagtacggc tgccatacat gaacgacgtg gcaagaatgt caaaggcaaa
      901 tggcaggggt cctccctcga tgactttgat cctcatggtt tgagttccca tgtacggatt
      961 gccttctcct tcacctgtgc atttgaattg gtggccgttg accgaacctt ccatgaccac
     1021 cttcatacgc
//
