LOCUS       sequence-140778-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""intermediate vector"" for the two-step cloning method (Wang and 
            Szaro, Dev Biol, 2015).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pBluescriptII[attB/Ins1]
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     278..294
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     279..295
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     337..353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        361..379
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     361..378
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      121 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      181 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
      241 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgattaa
      301 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattga
      361 atttaggtga cactatagaa gagctatgac gtcgcggtgc gggtgccagg ggcgtgccct
      421 tgacgtcgca tgcacgcgta cgtagacaga ctcatatccg cgggagctca cggggacagc
      481 ccccccccaa agcccccagg gctgtaattg cgttcctctc ccgctagggg gcagcagcga
      541 gccgcccagg gctccgctcc ggcccgacgc tccccccgca tccccgagcc ggagcccgca
      601 gcgtgcgggg acagcccggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
      661 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttatctaga
      721 tccgcgggag ctcacgggga cagccccccc ccaaagcccc cagggctgta attgcgttcc
      781 tctcccgcta gggggcagca gcgagccgcc cggggctccg ctccggcc
//
