LOCUS       sequence-140782-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS285 C terminal mKO2 hygBR
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..735
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg ctctagaggt
       61 gctggatctg gttcattaat taacatggtt tctgttatta agcctgaaat gaagatgcgt
      121 tactatatgg atggttctgt taatggtcat gaatttacta ttgaaggtga aggtactggt
      181 cgtccttatg aaggtcatca agaaatgact cttcgtgtta ctatggctga aggtggtcct
      241 atgccttttg cttttgatct tgtttctcat gttttttgct atggtcatcg tgtttttact
      301 aagtatcctg aagaaattcc tgattacttt aagcaagctt ttcctgaagg tctttcttgg
      361 gaacgttctc ttgaatttga agatggtggt tctgcttctg tttctgctca tatttctctt
      421 cgtggtaata ctttttacca taagtctaag tttactggtg ttaattttcc tgctgatggt
      481 cctattatgc aaaatcaatc tgttgattgg gaaccttcta ctgaaaagat tactgcttct
      541 gatggtgttc ttaagggtga tgttacaatg taccttaagt tagaaggtgg tggtaaccat
      601 aagtgccaaa tgaagactac ttacaaggct gctaaagaaa ttcttgaaat gcctggtgat
      661 cattacattg gacatcgtct tgttcgtaag actgaaggta acattactga acaagttgaa
      721 gatgctgttg ctcattattc ttaaaaaaag actaatgtaa aatttttttg gttggttatt
      781 gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca gaaggcattt
      841 agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa tattaactat
      901 tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt aaacatgctc
      961 ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
//
