LOCUS       sequence-140783-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS285 C terminal mKO2 hygBR
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..39
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     67..83
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     68..84
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(106..303)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc
       61 cccccctcga ggtcgacggt atcgataagc ttgatatgtt taaaccagta tagcgaccag
      121 cattcacata cgattgacgc atgatattac tttctgcgca cttaacttcg catctgggca
      181 gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca tttgattaaa atagaacaac
      241 tacaatataa aaaaactata caaatgacaa gttcttgaaa acaagaatct ttttattgtc
      301 agtactgatt attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
      361 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
      421 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
      481 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
      541 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
      601 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
      661 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
      721 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
      781 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
      841 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
      901 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
      961 gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
     1021 tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggt
//
