LOCUS       sequence-140784-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS285 C terminal mKO2 hygBR
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..216
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             217..1020
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt
       61 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
      121 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
      181 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
      241 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
      301 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
      361 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
      421 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
      481 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
      541 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
      601 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
      661 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
      721 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
      781 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
      841 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
      901 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
      961 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
     1021 aaa
//
