LOCUS       sequence-140785-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS285 C terminal mKO2 hygBR
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    79..582
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        619..723
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(942..961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat
      121 gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
      181 ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag
      241 gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca
      301 gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt
      361 gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag
      421 agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa
      481 acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt tatatattta
      541 tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc
      601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1021 ttggttga
//
