LOCUS       sequence-140789-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a CCR5TC binding site upstream of it, 
            driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) 
            repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     166..187
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             272..613
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             662..787
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     protein_bind    complement(795..819)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(845..864)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
       61 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      121 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      181 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtactcta ggggagctgg
      241 cgctggagcg ggtgcagggg ctggctctag attccagtgc cggatctgca tgcggaactt
      301 cagcgaccgg tccaacctga gcaggcacat cagaacccac accggagaaa agcccttcgc
      361 ctgcgacatt tgcggccgga agttcgccat cagcagcaac ctgaacagcc acaccaagat
      421 ccacactggc agccagaaac ctttccagtg cagaatttgt atgagaaact ttagcagaag
      481 cgacaacctg gccagacaca tccggacaca tactggtgaa aaaccttttg cctgtgatat
      541 ctgtggcaga aagtttgcca cctccggcaa tctgacccgg cacacaaaga ttcacctgcg
      601 gggcagccag ctatcgattg tcgacgctcc tgaacaacgt gaaggtgctt ctcaagtttc
      661 tgtttctgtt acttttgaag atgttgctgt tctttttact cgtgatgaat ggaaaaaact
      721 tgatctttct caacgttctc tttatcgtga agttatgctt gaaaattatt ctaatcttgc
      781 ttctatggct taatacccag ctttcttgta caaagtgggg gatccagaca tgataagata
      841 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaa
//
