LOCUS       sequence-140790-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a CCR5TC binding site upstream of it, 
            driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) 
            repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(80..91,92..254)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     627..643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     627..643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    663..683
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     685..704
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        685..703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(756..847)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caaaagacgt gaatatctgt tcagacaccc atatccactc tgttccacac aggtcagagg
       61 tttgtccagg agttcttgac agaggtgtaa aaagtactca aaaattttac tcaagtgaaa
      121 gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt
      181 acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta aggaagtaaa
      241 agtaaaagca agaaagaaaa ctagagattc ttgtttaagc ttttaatctc aaaaaacatt
      301 aaatgaaatg catacaaggt tttatcctgc tttagaactg tttgtattta attatcaaac
      361 tataagacag acaatctaat gccagtacac gctactcaaa gttgtaaaac ctcagattta
      421 acttcagtag aagctgattc tcaaaattgt tagtgtcaag cctagctctt ttggggctga
      481 aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc cgatgcggga agaggtgtat
      541 tagtcttgat agagaggctg caaatagcag gaaacgtgag cagagactcc ctggtgtctg
      601 aaacacaggc cagatgggcc ctcgagcagg aaacagctat gaccatgatt acgccaagct
      661 atcaactttg tatagaaaag ttggtaatac gactcactat agggcgaatt gggtaccaag
      721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      841 aaacaaatag gggttc
//
