LOCUS       sequence-140793-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Myr-mIFP in Zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: pCS2+_Myr-mIFP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    238..372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(262..281)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     316..335
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(485..505)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(485..503)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(524..540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(524..540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(537..559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    548..564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(572..578,579..596,597..602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    617..638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(755..772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgggcgttgg cgcgaccctg gtttgctctc tgatggtgtc tggtcgtctg tggggtctga
       61 tcgcttgcca ccactacgaa ccgcgcttcg ttccgttcca cattcgcgct gctggcgaag
      121 cgctggcgga aacttgtgcg atccgcatcg cgacgctgga gagctttgca cagtctcagt
      181 ccaaataaaa ttcaaggcct ctcgagcctc tagaactata gtgagtcgta ttacgtagat
      241 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      301 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      361 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      421 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt
      481 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
      541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta
      601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      901 gtaaaaaggc cgcgttgctg gcgtttttcc ata
//
