LOCUS       sequence-140795-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing of iBlueberry and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: iBlueberry T2A mCherry
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             192..245
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             252..968
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGA
                     LKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYE
                     RAEGRHSTGGMDELYK"
     primer_bind     complement(403..421)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     671..690
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1000..1017)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tgggcgttgg cgcgaccctg gtttgctctc tgatggtgtc tggtcgtctg tggggtctga
       61 tcgcttgcca ccactacgaa ccgcgcttcg ttccgttcca cattcgcgct gctggcgaag
      121 cgctggcgga aacttgtgcg atccgcatcg cgacgctgga gagctttgca cagtctcagt
      181 ccaaaggatc cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
      241 gcccagaatt catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      301 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      361 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      421 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      481 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      541 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      601 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      661 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      721 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      781 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      841 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      901 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      961 acaagtaatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     1021 gcagccatct gttgtttgcc cctccccgtg ccttcct
//
