LOCUS       sequence-140797-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing UAS-CD4-mIFP T2A HO1 in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: pACU2_CD4-mIFP T2A HO1
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             189..200
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             192..245
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgggcgttgg cgcgaccctg gtttgctctc tgatggtgtc tggtcgtctg tggggtctga
       61 tcgcttgcca ccactacgaa ccgcgcttcg ttccgttcca cattcgcgct gctggcgaag
      121 cgctggcgga aacttgtgcg atccgcatcg cgacgctgga gagctttgca cagtctcagt
      181 ccaaagatat cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
      241 gcccaatgga aagaccacag ccagactcta tgccacagga tctgagcgaa gccctcaagg
      301 aggccaccaa ggaagtgcac acccaggccg agaacgctga gttcatgaga aacttccaga
      361 aaggacaggt tactcgcgac ggattcaagc tggtgatggc tagcctctat cacatctacg
      421 tggccctgga ggaggaaatt gagagaaaca aggaatcccc cgtgttcgct ccagtttatt
      481 tcccagagga gctgcatcgg aaggccgccc tggaacagga tctggctttt tggtatggcc
      541 cccgctggca ggaggtcatt ccttataccc ccgctatgca acgctacgtt aagagactcc
      601 acgaggtggg ccgcacagaa cctgagctgc tcgtggccca tgcctacacc cgctacctgg
      661 gcgacctgtc agggggtcaa gttctgaaaa agatagccca aaaggccctg gacctgcctt
      721 cctctggcga ggggctggcc ttctttactt tccctaacat tgcctcagcc acaaagttca
      781 agcagctcta ccggtccagg atgaacagcc tggaaatgac cccagccgtg cggcagcggg
      841 tcatagaaga agccaagact gcctttctgc tgaatatcca gctgtttgag gaactccagg
      901 aactgctgac ccatgacact aaagaccagt ccccctccag ggccccagga ctcagacaga
      961 gggcctcaaa caaggttcag gactca
//
