LOCUS       sequence-14081-e        5160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Drews C, Senkel S, Ryffel GU
  TITLE     The nephrogenic potential of the transcription factors osr1, osr2, 
            hnf1b, lhx1 and pax8 assessed in Xenopus animal caps.
  JOURNAL   BMC Dev Biol. 2011 Jan 31;11:5. doi: 10.1186/1471-213X-11-5.
  PUBMED    21281489
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2011-01-31"; volume: "11:5. doi"; pages: " 
            10.1186/1471-213X-11-5"
COMMENT     Sequence Label: OSR1-pCS2+MT
FEATURES             Location/Qualifiers
     source          1..5160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1209..1343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1233..1252)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1287..1306
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1456..1476)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1456..1474)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1495..1511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1495..1511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1508..1530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1519..1535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1543..1549,1550..1567,1568..1573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1588..1609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1726..1743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1897..2485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1977..1996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2656..3447,3448..3516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3279..3298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3517..3621)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3647..4102)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3784..3805)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3996..4015
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4176..5160
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 cctctcgagc ctctagagat gggcagcaaa accttgccgg cgccggtgcc tatccaccct
      421 tccctgcagc tcaccaacta ctccttcctt caggcagtga acggcctgcc cacagtgcct
      481 tcggaccatc tgcccaacct gtatggtttc agcgcgttgc acgctgtgca cctgcatcag
      541 tggacgctgg gctacccggc catgcacttg ccgcgctctt ctttctccaa agtgccgggc
      601 acggtgtcca gcttggtgga tgcgcgcttc cagctgcccg cctttccctg gttccctcat
      661 gtcattcaac ccaagcccga gatcaccgct ggaggcagcg ttccagcgct caagaccaag
      721 ccgcgctttg attttgccaa cctggccttg gcagcaacgc aagaagatcc ggccaagctc
      781 ggtcgcgggg agggcccagg ctcccctgca ggtgggctgg gtgccctcct cgacgtgacc
      841 aagctgtctc cagaaaagaa gcccacaagg ggacgtctgc cttccaagac caagaaggaa
      901 ttcgtctgca agttctgtgg ccgccacttc accaagtcct acaacctact tatccatgag
      961 cggacgcaca ccgacgagcg gccctacacc tgtgacatct gccacaaagc cttccggagg
     1021 caagaccacc tgcgagacca cagatatatt cactccaaag agaagccctt caagtgtcaa
     1081 gagtgtggga aaggattctg ccagtccagg actctcgctg tccacaagac gctacactca
     1141 caggtgaagg agctcaaaac ctccaagatc aaatgctaat ctagaactat agtgagtcgt
     1201 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1261 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1321 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1381 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
     1441 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     1501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1561 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1681 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1741 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1801 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1861 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1921 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1981 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2041 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2101 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2161 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2221 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2281 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2341 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2401 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2461 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2521 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2581 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     3661 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3721 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3781 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3841 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3901 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3961 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4021 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4081 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     4141 cgatcggtgc gggcctcttc gctattacgc cagtcgacca tagccaattc aatatggcgt
     4201 atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt
     4261 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
     4321 acttggcacc atgccaattc aatatggcgg acttggcact gtgccaactg gggaggggtc
     4381 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
     4441 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
     4501 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
     4561 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
     4621 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
     4681 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
     4741 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
     4801 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
     4861 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
     4921 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa ggacgccagg
     4981 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
     5041 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
     5101 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac
//
