LOCUS       sequence-140814-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac 
            promoter, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pZ8-T_dCas9
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(439..444,445..460,461..467)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    475..491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     480..502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             517..582
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             589..609
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
       61 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
      121 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
      181 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
      241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      301 ccgactggaa agcgggcagt gactgtgcag gtcgtaaatc actgcataat tcgtgtcgct
      361 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
      421 atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga
      481 gcggataaca atttcacaca ggaaacagaa ttcatggact acaaagacca tgacggtgat
      541 tataaagatc atgacatcga ttacaaggat gacgatgaca agatggcccc caagaagaag
      601 aggaaggtgg gccgcggaat ggataagaaa tactcaatag gcttagctat cggcacaaat
      661 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
      721 ctgggaaata cagaccgcca cagtatca
//
