LOCUS       sequence-140815-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pyk (T) sgRNA targeting the template 
            strand of pyk, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pyk_T
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        98..173
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttgcgccgac atcataacgg ttctggcaaa tattcgaaat gagctttgac aattaacatc
       61 ggctcgtata atgagattgt atgtacccta ggcccaggtt ttagagctag aaatagcaag
      121 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta
      181 tggaattgga tccgtggaag acatctgtcg aagacacctt gtggtcgaag acaccttgtc
      241 tcttcccaga ggcgtggtcg catactaatt tgtaaacggc catgacgaaa tagtcgtacg
      301 gaacaaggag ccactagtct agaattcggt gaggttatgg cggagggttg cgaggtctag
      361 gagaacagag gaagtcatgc tttgaagcat ataagctgcc ctgcccctca aggttttctt
      421 caagtgaggt tttatctaac tgcctaacgg caggggaacc gtatattgct tacggtatga
      481 gaccccttaa acgtccggat agtcaccgct cttctttagc tccgcgacat gcctagcaac
      541 cgtggcgcga gagactccta cctctgcccc tatttcagcc cacgtgggaa ctgtccctgt
      601 ctggaaatac tgatcgttca ccatttggct aatacgggac ttcgtagatc gtccttgagc
      661 ctttttctta cggtgcgtct tttcaagctt cgacctttgt gcttgcgcat atttgccctc
      721 ggggtctgtt ttccagcgtt gtgcggcttt ttgtccgcct ctgcgtccca tcgtggccaa
      781 ggctttccgc tcgctgctgg tggctttacc tggtgcgtta gagccgctgt aggtctcgct
      841 cttggattgg gcgacatacc cgcgcacgcg ccttgccatg g
//
