LOCUS       sequence-140817-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the 
            non-template strand of pgi, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pgi_NT
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(43..47,48..63,64..70)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        95..170
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgacatcata acggttctgg caaatattct gaaatgagct gctgacaatt aatcatcggc
       61 tcgtataatg ttgcctggaa gtttgagtaa tgatgtttta gagctagaaa tagcaagtta
      121 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttatgg
      181 aattggatcc gtggaagaca tctgtcgaag acaccttgtg gtcgaagaca ccttgtctct
      241 tcccagaggc gtggtcgcat actaatttgt aaacggccat gacgaaatag tcgtacggaa
      301 caaggagcca ctagtctaga attcggtgag gttatggcgg agggttgcga ggtctaggag
      361 aacagaggaa gtcatgcttt gaagcatata agctgccctg cccctcaagg ttttcttcaa
      421 gtgaggtttt atctaactgc ctaacggcag gggaaccgta tattgcttac ggtatgagac
      481 cccttaaacg tccggatagt caccgctctt ctttagctcc gcgacatgcc tagcaaccgt
      541 ggcgcgagag actcctacct ctgcccctat ttcagcccac gtgggaactg tccctgtctg
      601 gaaatactga tcgttcacca tttggctaat acgggacttc gtagatcgtc cttgagcctt
      661 tttcttacgg tgcgtctttt caagcttcga cctttgtgct tgcgcatatt tgccctcggg
      721 gtctgttttc cagcgttgtg cggctttttg tccgcctctg cgtcccatcg tggccaaggc
      781 tttccgctcg ctgctggtgg ctttacctgg tgcgttagag ccgctgtagg tctcgctctt
      841 ggattgggcg acatacccgc gcacgcgcct tgccatggtt
//
