LOCUS       sequence-140819-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant 
            E162A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Duong-Ly KC, Woo HN, Dunn CA, Xu W, Babic A, Bessman MJ, Amzel LM, 
            Gabelli SB
  TITLE     A UDP-X diphosphatase from Streptococcus pneumoniae hydrolyzes 
            precursors of peptidoglycan biosynthesis.
  JOURNAL   PLoS One. 2013 May 15;8(5):e64241. doi: 
            10.1371/journal.pone.0064241. Print 2013.
  PUBMED    23691178
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-15"; volume: "8(5):e64241. doi"; pages: " 
            10.1371/journal.pone.0064241. Print 2013"
COMMENT     Sequence Label: pET24a_UDP_Xase_E162
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             680..697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(750..768)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctttaagaag gagatataca tatgaagaca agtgattttg tcaagtattt gcaaagaatg
       61 attgccatta cagatactgg cttaaccttt acaaaagatc cgtttgaccg tgagcgctac
      121 gaagacttgc gaagtctgtt atctgaaatg ttgaatcaag catcagacct tgattccgaa
      181 gaagtggcag aagtcttgaa gccaacttct gcttatgcga ctccgttaat ggacgtccgt
      241 gcttggattg ttgaggatga gaagatttgt ctggttaggg gacaaggaga ggatagttgg
      301 gctttgccag gtggctttgg tgaagtcggc tattctccaa cagaaaatat tctcaaggaa
      361 attgaagaag aaaccggttt taaagccaaa gttgaaagac tgctagctgt ttttgatacc
      421 aatcgtttcc aactacagag caaacagtat acaaagtttg tttttggatg taagcttctt
      481 gatggacaat tccaagaaaa tcaagcaatt gctgaccttc aattttttgc cattgaccaa
      541 ctgccgaact tatctgaaaa acgcattacc aaggagcaaa tagagcttct ttggcaggtt
      601 tatcaaggtc atagggggca atatcttgac taaggatccg aattcgagct ccgtcgacaa
      661 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      721 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
//
