LOCUS       sequence-140820-         281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible 
            prpD2 promoter (PprpD2), KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pZ8-P_dCas9
FEATURES             Location/Qualifiers
     source          1..281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..58)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acggatcccc gggaattctg tttcctgtgt gtcagcccac cttcctcttc ttcttggggt
       61 cacctcctag ctgactcaaa tcaatgcgtg tttcataaag accagtgatg gattgatgga
      121 taagagtggc atctaaaact tcttttgtag acgtatatcg tttacgatca attgttgtat
      181 caaaatattt aaaagcagcg ggagctccaa gattcgtcna cgtnaataaa tgaataatat
      241 tttctgcttg ttcacgtatt ggtttgtctc tatgtttgtt a
//
