LOCUS       sequence-140821-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible 
            prpD2 promoter (PprpD2), KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pZ8-P_dCas9
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             295..360
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             367..387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acatcataac ggttctggca aatattctga aatgagctgc tccagcgtcc aagaatatgc
       61 ccccgcgcgc cgggtgggga gcgaagggaa cccccaagga attggcgttg aggtggtgat
      121 tttgcatgtt ttactcaaaa tcactttgat ggtcacaaaa ttacacaact tttacagtga
      181 cctacattgc tttttaaaga attagtgtgg tgtgcgatcc ccgggtaccc agcttgagta
      241 ggacaaatcc gccgagcttc gacgagattt tcaggagcta aggaagctaa aatggactac
      301 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
      361 atggccccca agaagaagag gaaggtgggc cgcggaatgg ataagaaata ctcaataggc
      421 ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
      481 tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata
      541 ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa acggacagct
      601 cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat
      661 gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt tttggtggaa
      721 gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga agttgcttat
      781 catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc tactgataaa
      841 gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg tggtcat
//
