LOCUS       sequence-140825-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Antares in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Chu J, Oh Y, Sens A, Ataie N, Dana H, Macklin JJ, Laviv T, Welf ES, 
            Dean KM, Zhang F, Kim BB, Tang CT, Hu M, Baird MA, Davidson MW, Kay 
            MA, Fiolka R, Yasuda R, Kim DS, Ng HL, Lin MZ
  TITLE     A bright cyan-excitable orange fluorescent protein facilitates 
            dual-emission microscopy and enhances bioluminescence imaging in 
            vivo.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3550.
  PUBMED    27240196
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3550."
COMMENT     Sequence Label: pNCS-Antares
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cctgatgcga tgaatgatat aataggaaag tactgttttg tttaacttta agaaggggat
       61 atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgatggt gagcaagggc
      181 gaggagctga tcaaggagaa catgagaagc aagctgtacc tggaaggcag cgtgaacggc
      241 caccagttca agtgcaccca cgaaggggag ggcaagccct acgagggcaa gcagaccaac
      301 aggatcaagg tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacccac
      361 tttatgtacg ggagcaaggt gttcatcaag taccccgccg acctccccga ttattttaag
      421 cagtccttcc ctgagggctt cacatgggag agagtcatgg tgttcgaaga cgggggcgtg
      481 ctgaccgcca cccaggacac cagcctccag gacggcgagc tcatctacaa cgtcaaggtc
      541 agaggggtga acttcccagc caacggcccc gtgatgcaga agaaaacact gggctgggag
      601 cccagcaccg agaccatgta ccccgctgac ggcggcctgg aaggcagatg cgacaaggcc
      661 ctgaagctcg tgggcggggg ccacctgcac gtcaacttca agaccacata caagtccaag
      721 aaacccgtga agatgcccgg cgtccactac gtggaccgca gactggaaag aatcaaggag
      781 gccgacaacg agacctacgt cgagcagtac gagcacgctg tggccagata ctccaacctg
      841 ggcggaggct tcacactcga
//
