LOCUS       sequence-140828-        3233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Photoconvertible eos in a middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Prendergast A, Linbo TH, Swarts T, Ungos JM, McGraw HF, Krispin S, 
            Weinstein BM, Raible DW
  TITLE     The metalloproteinase inhibitor Reck is essential for zebrafish DRG 
            development.
  JOURNAL   Development. 2012 Mar;139(6):1141-52. doi: 10.1242/dev.072439. Epub 
            2012 Feb 1.
  PUBMED    22296847
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.072439"; journalName: "Development"; date: "2012-03"; 
            volume: "139"; issue: "6"; pages: "1141-52"
COMMENT     Sequence Label: pME eos
FEATURES             Location/Qualifiers
     source          1..3233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             677..1357
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     protein_bind    complement(1358..1457)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1474..1493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1475..1493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1498..1514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1498..1514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1616..1635)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1627..2436
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1694..1713)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2583..3171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3072..3091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc gccaccatga gtgcgattaa gccagacatg aagatcaacc tccgtatgga
      721 aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga
      781 gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga
      841 tatcctgacc actgcattcc attacggcaa cagggtattc gccgaatatc cagaccacat
      901 acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt
      961 cgaagacggg ggcatttgca ttgccagaaa cgacataaca atggaagggg acactttcta
     1021 taataaagtt cgatttcacg gtgtaaactt tcccgccaat ggtccagtta tgcagaagaa
     1081 gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg
     1141 tgatattacc atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac
     1201 tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg tggaccactg
     1261 cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt
     1321 tgctcattct ggattgcctg acaatgccag acgatagacc cagctttctt gtacaaagtt
     1381 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1441 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1501 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1561 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1621 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1681 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1741 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1801 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1861 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1921 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1981 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2041 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2101 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2161 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2221 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2281 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2341 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2401 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2461 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2521 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2701 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
