LOCUS       sequence-140833-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of mClover3 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pNCS-mClover3
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             111..143
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(147..155,156..170)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(222..243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tattcttgac acctgatgcg atgaatgata taataggaaa gtactgtttt gtttaacttt
       61 aagaagggga tatacatatg cggggttctc atcatcatca tcatcatggt atggctagca
      121 tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgatgg
      181 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      241 acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga gggcgatgcc accaacggca
      301 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      361 tgaccacctt cggctacggc gtggcctgct tcagccgcta ccccgaccac atgaagcagc
      421 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctctttca
      481 aggacgacgg tacctacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      541 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      601 tggagtacaa cttcaacagc cactacgtct atatcacggc cgacaagcag aagaactgca
      661 tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg cagcgtgcag ctcgccgacc
      721 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      781 tgagccatca gtccaagctg agcaaagacc
//
