LOCUS       sequence-140848-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid driven under neuronal beta-tubulin promoter, bearing attB 
            and two insulator sequences (opposite directions pointing inward 
            towards the reporter gene).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin
            3...
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     84..103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        84..102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     127..143
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     128..144
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        210..228
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     210..227
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctgcaagggc tagccgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      121 cccccctcga ggtcgacggt atcgattaag ttgggtaacg ccagggtttt cccagtcacg
      181 acgttgtaaa acgacggcca gtgaattgaa tttaggtgac actatagaag agctatgacg
      241 tcgcggtgcg ggtgccaggg gcgtgccctt gacgtcgcat gcacgcgtac gtagacagac
      301 tcatatccgc gggagctcac ggggacagcc ccccccccaa agcccccagg gctgtaattg
      361 cgttcctctc ccgctagggg gcggcagcga gccgcccagg gctccgctcc ggcccgacgc
      421 tccccccgca tccccgagcc ggagcccgca gcgtgcgggg acagcccggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaagc tttatctaga tccgcgggag ctcacgggga cagccccccc
      601 cc
//
