LOCUS       sequence-140850-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector bearing an NheI restriction site for the two-step cloning 
            method in Wang and Szaro, Dev Biol, 2015.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
       61 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      121 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaagggc tagccgccac
      181 catgagcaag ggcgaggaac tgttcactgg cgtggtccca attctcgtgg aactggatgg
      241 cgatgtgaat gggcacaaat tttctgtcag cggagagggt gaaggtgatg ccacatacgg
      301 aaagctcacc ctgaaattca tctgcaccac tggaaagctc cctgtgccat ggccaacact
      361 ggtcactacc ttcacctatg gcgtgcagtg cttttccaga tacccagacc atatgaagca
      421 gcatgacttt ttcaagagcg ccatgcccga gggctatgtg caggagagaa ccatcttttt
      481 caaagatgac gggaactaca agacccgcgc tgaagtcaag ttcgaaggtg acaccctggt
      541 gaatagaatc gagctgaagg gcattgactt taaggaggat ggaaacattc tcggccacaa
      601 gctggaatac aactataact cccacaatgt gtacatcatg gccgacaagc aaaagaatgg
      661 catcaaggtc aacttcaaga tcagacacaa cattgaggat ggatccgtgc agctggccga
      721 ccattatcaa cagaacactc caatcggcga cggccctgtg ctcctcccag acaaccatta
      781 cctgtccacc cagtctgccc tgtctaaaga tcccaacgaa aagagagacc acatggtcct
      841 gctggagttt gtgaccgctg ctgggatcac acatggcatg gacgagctgt acaagtgagc
      901 ggccgcggat cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt
      961 cgctattg
//
