LOCUS       sequence-140853-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector bearing an NheI restriction site for the two-step cloning 
            method in Wang and Szaro, Dev Biol, 2015.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        80..157
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(177..196)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ccgcctctcc ccgcgcgttg gccgattcat taatgcagag cttgcaattc gcgcgcgaag
       61 gcgaagcggc atttacgttg acaccatcga atggcgcaaa acctttcgcg gtatggcatg
      121 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
      181 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
      241 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
      301 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
      361 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
      421 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
      481 agcggcggtg cacaatcttc tcgcgcaacg ggtcagtggg ctgatcatta actatccgct
      541 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
      601 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
      661 actgggcgtg gagcatctgg tcgcattggg ttaccactta gacagactca tatccgcggg
      721 agctcacggg gacagccccc ccccaaagcc ccctgggctg taattgcgtt cctctcccgc
      781 tagggggcag cagcgagccg cccggggctc cgctccggcc cgacgctccc cccgcatccc
      841 cgagccggag cccgcagcgt gcggggacag cccggcacgg ggaaggtggc acgggatcgc
      901 tttcctctga acgcttctcg ctgctctttg agcctgcaga cac
//
